BENEFICIARY 03 - UCBL

BRIEF DESCRIPTION OF THE ORGANIZATION   

The Laboratory of Analytical Sciences (LSA) laboratory is a JRU (Joint Research Unit according to the European Commission or UMR according to the French Research Ministry), and its legal representatives are Claude Bernard University – Lyon 1 (UCBL) and CNRS (National Scientific Research Centre. The scientific expertise of the UMR CNRS 5180 is focussed onto basic research in analytical chemistry and its applications in industrial processes, environment and biomedical. The permanent staff of the unit is composed of 26 researchers and teaching/researchers, 16 engineers and technicians. In addition, 40 doctoral students, post-doctoral students and invited professors are working in the unit.

The activity of the group “Surfaces, Nanotechnology and Biosensors” is mainly devoted to the conception and characterization of based NMT (bio)chemical sensors. The aim is to conceive and to engineer surfaces and layers, including recognizing molecules, well adapted to various transducers (FETs based, microconductimetric, impedancemetric, etc.).

The following technological areas are studied: 1) surface preparation by plasma techniques, 2) surface functionalization with molecules bearing active groups using covalent coupling, 3) self-assembled or Langmuir-Blodgett layers, 4) coupling of biospecies (enzymes, antibodies), 5) handling and biofunctionalization of nanoparticles. Characterization methods such as XPS, AES, IS, FTIR, SEM, AFM, TOF-SIMS, EPMA, XRFS, LEEIXS, impedance spectroscopy (EIS), electrochemical techniques (CV ..), and contact angle and tensiometer are available within the group.

MAIN TASKS WITHIN THE PROJECT

In Bond Project, the group “Surfaces, Nanotechnology and Biosensors” of Laboratory of Analytical Sciences is involved in the irreversible grafting of specific antibody on 3D nanoobjects including gold nanoparticles grafted on magnetic nanoparticles, this new approach being proposed by this group in order to increase the impedancemetric detection of related odorant of the immunorecognized olfactory receptor. This group will also be involved in the validation of every olfactory nanosensor in synthetic media and in real media.

The group “Surfaces, Nanotechnology and Biosensors” of Laboratory of Analytical Sciences has a strong background in the irreversible grafting of antibodies on gold surfaces and the specific recognition of olfactory receptors included in nanosomes acquired in the framework of european and national projects (SPOT-NOSED and BIOSENSEUR OLFACTIF). Moreover this group detected, for the first time, the related odorant using the label-free technique, electrochemical impedance spectroscopy (EIS). In BOND project, the specific antibody will be irreversibly grafted on 3D nanoobjects including gold nanoparticles grafted on magnetic nanoparticles. The use of magnetic nanoparticles functionalized with antibodies, allows on one hand the purification of species of interest and on the other hand their concentration on a microelectrode, simply by using a magnet. Moreover the group “Surfaces, Nanotechnology and Biosensors” of Laboratory of Analytical Sciences have used streptavidin fonctionalized magnetic nanoparticles based biosensor for E.coli detection and have compared this system with the self-assembled multilayer system described in a previous work. The sensitivity of the biosensor resulting of the immobilization of the magnetic nanoparticles on the microelectrode surface, was improved by a factor of three, that is due largely to the specific area of the magnetic nanoparticles which provide high capturing for target biomolecules. One of the new approach in BOND project is the improvement of the response of the impedancemetric measurement by the immobilization of the specific antibodies against olfactory receptor on gold nanoparticles. The specific surface area and the electrical properties of these particles will allow the improvement of the impedancemetric signal as it has been recently demonstrated, where presence of gold nanoparticles allows a decrease of the detection limit by a factor of 100 compared to previous results without gold nanoparticles. The group “Surfaces, Nanotechnology and Biosensors” of Laboratory of Analytical Sciences is now experienced in the immobilization of antibodies on gold nanoparticles and its application in EIS and SPR detection.

PREVIOUS EXPERIENCE

The group “Surfaces, Nanotechnology and Biosensors” is involved in FP5 and FP6 European projects, in bilateral international projects, in regional and national projects in different application domains: environmental monitoring, health care, industrial processes. The group has a strong background in the irreversible grafting of antibodies on gold surfaces and the specific recognition of olfactory receptors included in nanosomes acquired in the framework of european and national projects (SPOT-NOSED and BIOSENSEUR OLFACTIF).

CONTACT


Nicole JAFFREZIC-RENAULT
Director of Research at the Centre National de la Recherche Scientifique (CNRS)

UMR5180 Sciences Analytiques
Batiment CPE - 43, Boulevard du 11 novembre 1918
69622 Villeurbanne (France)
Tel. +33 472 438 306
Fax. +33 472 431 206
Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Web: http://lsa.univ-lyon1.fr/user/default.asp

KEY PEOPLE   

Nicole Jaffrezic-Renault received her engineering degree from the Ecole Nationale Supérieure de Chimie, Paris, France in 1971 and the Doctorat d’Etat és Sciences Physiques from the University of Paris in 1976. She joined Ecole Centrale de Lyon, France in 1984 and Claude Bernard University Lyon 1 in 2007. She is Director of Research at the Centre National de la Recherche Scientifique and the president of the chemical micro sensor club (CMC2). Her research activities include the design and fabrication of new biosensors based on biocatalytic systems and affinity systems and on electrochemical transduction. Label-free detection and miniaturization for integration in lab-on-chips are the main directions for this research. These biosensors are developed for in situ monitoring in biological fluids, natural waters and in food stuffs. Several technogy transfers to industrial companies were performed.


Abdelhamid Errachid graduated in Physics from the University M. Ismail, Meknes, 1992, and received a PhD degree in Electronic Engineering (with honors) from the Universitat Autònoma de Barcelona, Spain, in 1998. From 1998 to 2001 he worked at the Centro Nacional de Microelectrónica (CNM) as researcher. At the end of 2001, he joined the Research Centre for BioElectronics and NanoBioScience (CBEN) at the University of Barcelona, as researcher within the Spanish Ramon y Cajal Program. He has been working in the design, fabrication and characterisation of chemical- and bio- sensors based on microelectrodes and FET (Field-Effect-Transistor) and in the development of integrated instrumentation for ISFETs (Ion-sensitive Field-Effect-Transistor). He has been involved in several European Projects (DEMAC, BARMINT, MICRO-CARD, MICRO-TRANS, SPOT-NOSED, MAPTECH, DVT-IMP). From 2006 to 2008 he was working as a senior researcher at the Institute for Bioengineering of Catalonia (IBEC) at the University of Barcelona. He joined Claude Bernard University-Lyon 1 as a Professor. His current research activity is focussed in BioElectronics, Biofonctionalisation and NanoBiotechnology.